Esterificação do ácido cinâmico usando catálise enzimática

Abstract

The formation of an ecological conscience and the obligation to preserve the environment, have generated the development of new methods for obtaining natural products, without causing damage to the environment, in view of the possibility of alternative control. Among the compounds of natural origin are cinnamic acid and its derivatives, which are easily found in fruits, flowers and vegetables. It has been explored due to its wide diversity in biological activities such as, antimicrobial and antifungal, antitumor activity against malignant human tumors, among others. In order to obtain these products in a way that does not harm the environment, the use of biocatalysts has been a clean alternative. And the application of enzymes as catalysts in chemical syntheses has gained great prominence in recent years, since its use has been promising and considered a clean source for the environment due to stimulating technological development with the use of renewable raw materials and with environmental preservation. Therefore, this work aims to synthesize, characterize and optimize the reaction conditions in the esterification of cinnamic acid, with the use of enzymatic catalysis. In this context, the results obtained indicate a great potential for the use of biocatalysts in the synthesis of esters, highlighting the enzyme lipase B of Candida antarctica (CALB), which reached values above 70%. In addition, the chromatogram analyzes indicated hexane as a good solvent for this type of reaction and the most appropriate molar ratio of 0.1: 0.5. Therefore, this work demonstrated the best conditions for the synthesis of esters.